Is phototoxicity compromising experimental results?
Light-induced damage to biological samples during fluorescence imaging is known to occur but receives too little attention by researchers. Read more
Light-induced damage to biological samples during fluorescence imaging is known to occur but receives too little attention by researchers. Read more
Our very first webinar is now live. The topic is “Fluorescent protein and sensors: A practical discussion” and you can register to view it at www.nature.com/webcasts/fluorescent_proteins. Read more
Stefan Hell and colleagues propose that the use of pulsed excitation with a delay of at least one microsecond between pulses can dramatically reduce photobleaching and increase the effective fluorescence signal in both single and multi-photon fluorescence microscopy. Is this something you would consider trying in your lab? Do you think the described benefits are worth the necessary equipment changes? Read more
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Let’s give statistics the attention it deserves
Let’s give statistics the attention it deserves
Guidelines for algorithms and software in Nature Methods