Is phototoxicity compromising experimental results?
Light-induced damage to biological samples during fluorescence imaging is known to occur but receives too little attention by researchers. Read more
Fluorescent Proteins and Sensors Webinar – Questions & Answers
Our very first webinar is now live. The topic is “Fluorescent protein and sensors: A practical discussion” and you can register to view it at www.nature.com/webcasts/fluorescent_proteins. Read more
Increasing your fluorescence signal using pulsed excitation
Stefan Hell and colleagues propose that the use of pulsed excitation with a delay of at least one microsecond between pulses can dramatically reduce photobleaching and increase the effective fluorescence signal in both single and multi-photon fluorescence microscopy. Is this something you would consider trying in your lab? Do you think the described benefits are worth the necessary equipment changes? Read more
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Let’s give statistics the attention it deserves
Let’s give statistics the attention it deserves
Guidelines for algorithms and software in Nature Methods