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Archive by tag | fluorescence

23 Nov 2013 | 12:06 PM

Is phototoxicity compromising experimental results?

Posted by Daniel Evanko | Categories: Biophysics, Editorials, Microscopy & Imaging

Light-induced damage to biological samples during fluorescence imaging is known to occur but receives too little attention by researchers.  Read more

Tags:

  • fluorescence
  • fluorescence microscopy
  • light-sheet microscopy
  • optogenetics
  • photodamage
  • phototoxicity
  • super-resolution

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17 Dec 2012 | 12:00 PM

Fluorescent Proteins and Sensors Webinar – Questions & Answers

Posted by Daniel Evanko | Categories: Microscopy & Imaging, Webcasts

Robert Campbell, David Piston and Thomas Knopfel

Our very first webinar is now live. The topic is “Fluorescent protein and sensors: A practical discussion” and you can register to view it at www.nature.com/webcasts/fluorescent_proteins.  Read more

Tags:

  • fluorescence
  • fluorescence microscopy
  • fluorescent proteins
  • fluorescent sensors

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10 Dec 2006 | 4:38 PM

Increasing your fluorescence signal using pulsed excitation

Posted by Daniel Evanko | Categories: Microscopy & Imaging, Nature Methods papers

Stefan Hell and colleagues propose that the use of pulsed excitation with a delay of at least one microsecond between pulses can dramatically reduce photobleaching and increase the effective fluorescence signal in both single and multi-photon fluorescence microscopy. Is this something you would consider trying in your lab? Do you think the described benefits are worth the necessary equipment changes?  Read more

Tags:

  • fluorescence

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