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Nucleic acid based molecular biology

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Nature Protocols

Generation of a double-fluorescent double-selectable Cre/loxP indicator vector for monitoring of intracellular recombination events »

Touchdown PCR for increased specificity and sensitivity in PCR amplification »

PCR-based gene targeting in Candida albicans »

PCR-amplification of GC-rich regions: 'slowdown PCR' »

A universal real-time PCR assay for the quantification of group-M HIV-1 proviral load »

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Protocols Network

Immunohistochemistry and In Situ Hybridization Protocols »

Identifying novel p53-target genes and their functional analysis »

Assessment of cry1Ab transgene cassette in commercial Bt corn MON810: Gene, Event, Construct & GMO specific concurrent characterization »

Reconstituted nucleosomes containing ubiquitinated histones »

Ubp-M knockdown, RT-PCR, and ChIP assays »

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