In June, Nature Methods focuses on the latest trends in single-molecule analysis.

Leaders in the field review the methodologies allowing to examine the mechanism of action of fundamental biological processes molecule by molecule. The Review and Perspective articles also provide practical guidance for some of the techniques that are most integral to in vitro single-molecule experiments.

They cover single-molecule fluorescence methods, microfluidic flow cells to manipulate experimental conditions during experiments, and force spectroscopy techniques such as atomic force and optical traps to manipulate individual molecules.

In addition, two timely original research articles in the same issue describe complementary advances in nanoscale visualization.

All these articles are accessible free of charge for the entire month of June. Check them out!