How do you handle terabytes of data? That is a question that more and more investigators must face, on a weekly basis.

Are you one of them? Light-sheet fluorescence imaging, for example, generates so much data in each experimental run that handling and storing the raw data is a challenge. Next-generation sequencing is another, much more ubiquitous, case.

Read the July issue editorial “Byte-ing off more than you can chew” and let us know about your own experience, problems and practical (or impractical) solutions.

Posted by Veronique Kiermer at 01:00 PM | Permalink | Comments (1) | TrackBacks (0)

Stefan Hell and colleagues propose that the use of pulsed excitation with a delay of at least one microsecond between pulses can dramatically reduce photobleaching and increase the effective fluorescence signal in both single and multi-photon fluorescence microscopy. Is this something you would consider trying in your lab? Do you think the described benefits are worth the necessary equipment changes?

Read the paper here and then add your opinion.

Posted by Daniel Evanko at 04:38 PM | Permalink | Comments (3) | TrackBacks (0)