Main

July 10, 2009

PRIDE Converter – A new tool for proteomics

The administrators of the EBI proteomics repository PRIDE have just announced, in the July issue of Nature Biotechnology, the availability of a software tool to facilitate data deposition.

According to Lennart Martens and co-authors, the new tool, PRIDE Converter, “makes it straightforward to submit proteomics data to PRIDE from most common data formats.” This comes handy.

Depositing proteomics data into a structured public repository is a very worthwhile effort—one that Nature Biotechnology, Nature Methods, and other Nature journals strongly encourage. To date, however, the problem has been that in some cases, depositing data remained an effort.

Things could get particularly challenging if you happened to have large datasets in instrument-specific formats. Converting these into the XML-based format needed for data submission required time and informatics skills. Apparently, now, PRIDE Converter does it for you.

Proteomics researchers need effective ways of sharing data. Submission of data to public repositories upon publication should become as automatic in proteomics as it is in some other fields. But realistically, this can only happen if a good infrastructure of databases is in place—it is—and if the submission process is not an undue burden on the researchers.

Kudos to PRIDE, thus, for taking this step in the right direction and for demonstrating a willingness to work with researchers to facilitate submission. It is up to proteomics researchers now, to make use of the tool, and work with its authors to continuously improve it as the field, and their needs, evolve.

February 28, 2008

Proteomics data deposition to public repositories

Starting in March, Nature Methods strongly recommends deposition of proteomics data to public repositories before manuscript submission. An Editorial in the March issue describes the motivation of this decision and comments on the public repositories that are now available to proteomics researchers.

We welcome your comments on this move.

September 27, 2007

Mass spectrometry in focus

Mass spectrometry has become the most powerful tool for proteomics, owing to its high-throughput capacity and molecular information yield. However, it is perhaps under-utilized in more traditional cell biology research, since historically, it was a tool developed by and for chemists. Our October issue celebrates this technology and its established and emerging biological applications with a special Focus on mass spectrometry in proteomics applications.

June 06, 2007

The quest for the antibody grail

One of the most common frustrations among biologists is the difficulty to get their hands on a good antibody with a decent chance to work well in the particular assay they have in mind.

There is not one universal quality control test for antibodies and many commercial antibodies which are advertised to work well, say, in Western blot will perform poorly in immunoprecipitation and abysmally in a FACS machine. Examples abound and researchers often lament about the money spent on re-testing antibodies or repeating experiments that have failed due to poor antibody quality.

Continue reading "The quest for the antibody grail" »

Welcome to methagora, Nature Methods’s commenting forum. Check here regularly for discussions on methodological topics of interest to your community. If you have a question or comment for the Nature Methods team or wish to propose a discussion topic, please e-mail methagora at natureny.com
Powered by
Movable Type 3.2