Nature Protocols | Stepwise

CAUTION: Don’t mix concentrated nitric acid with organic solvents!

Radiolabelling with copper-64 (or any other metal cation radioisotope) is done by attaching a metal chelating group to the probe of interest. In 2006, we worked with Thaddeus J Wadas & Carolyn J Anderson to publish a protocol for radiolabelling peptides with copper-64 which included a procedure for making sure that the reaction tubes and pippette tips used for the labelling were free from any other metals that might compete with copper-64 for the space in the chelator (Box 1 in the protocol). This procedure involved washing the equipment with nitric acid, followed by rinses with ethanol followed by diethyl ether. 

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