I asked veteran Nature reporter David Cyranoski what he had to leave out of his recent article that might interest Nature Reports Stem Cells Readers. Here’s what he had to say:
Hoechst, but they tried to minimize the UV exposure by using a very narrow
beam focused on just where they expected teh spindle to be. They did not
expose the whole cell. So, they did try to limit damage that Mitalipov was
worried about. Also, they didnt take too much cytopolasm or time.
If the first polar body wasn’t in the near vicinity fo the spindle, they left
it in there. He [interview at Stemagen] said that there is no concern of the first polar body
creating a parthenote unless it is injected (ie, it cant merely fuse). He
focused on getting the whole thing over with very quickly. There seems to be
a trade-off between trying to treat the cell nicely (by not using Hoechst,
eg) and trying to do it quickly (use Hoechst, but get in and out).