Mitochondria are energy-generating organelles that carry a portion of their own DNA. Mutations in mitochondrial proteins are associated with metabolic disorders, neurodegenerative diseases and a variety of malignancies. Joshua Coon, David Pagliarini and their colleagues generated proteomic, lipidomic and metabolomic data on 174 strains of yeast, each carrying a mutation in a different protein implicated in mitochondrial function. In their paper in the November issue of Nature Biotechnology, they show how connections among these different data sets can be exploited to reveal new insights into the biosynthesis of coenzyme Q, an essential lipid required for oxidative phosphorylation. Their paper can be found here.