Last December we published an Article by Simon Davis and colleagues challenging the conventional methodology being used in BRET studies that detect dimerization of G protein coupled receptors. The conclusions were contested in a Correspondence by Michel Bouvier and colleagues. In a new Correspondence, a former member of the Bouvier lab further argues against the results and conclusions of the Davis study with experimental results using the ‘Type 2’ BRET assay of Davis and colleagues.

Continue reading →

Using a new quantitative framework for evaluation of receptor dimerization using bioluminescence energy transfer (BRET), Simon Davis and colleagues at the University of Oxford present evidence that the signals arising from BRET measurements, and interpreted as evidence of dimerization, are the result of random interactions and not evidence of stable complexes.

Continue reading →