Nature Methods | Methagora

Light sheet imaging in Nature Methods

It was only a few months before Nature Methods was launched in October 2004 that Jan Huisken and Ernst Stelzer had published a paper in Science in which they used light sheet microscopy – what they called selective plane illumination microscopy or SPIM – to image fluorescence within transgenic embryos. Simplistically put, this century-old technique achieves optical sectioning by illuminating a sample through its width with a thin sheet of light. In the last decade, Nature Methods has published a steady stream of papers reporting developments in light-sheet imaging. Here are the highlights. 

BACK TO ARTICLE

Tell a Friend

Please ensure that your friend is content to receive an email from us with a link to the article. Select the following link to view our Privacy Policy.

BACK TO ARTICLE