Nature Methods | Methagora
Increasing your fluorescence signal using pulsed excitation
Stefan Hell and colleagues propose that the use of pulsed excitation with a delay of at least one microsecond between pulses can dramatically reduce photobleaching and increase the effective fluorescence signal in both single and multi-photon fluorescence microscopy. Is this something you would consider trying in your lab? Do you think the described benefits are worth the necessary equipment changes?
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Let’s give statistics the attention it deserves
Let’s give statistics the attention it deserves
Guidelines for algorithms and software in Nature Methods