{"id":65,"date":"2009-08-09T17:07:46","date_gmt":"2009-08-09T17:07:46","guid":{"rendered":"https:\/\/blogs.nature.com\/methagora\/2009\/08\/top-downloads-for-july-09.html"},"modified":"2011-12-22T15:26:58","modified_gmt":"2011-12-22T15:26:58","slug":"top_downloads_for_july_09_1","status":"publish","type":"post","link":"https:\/\/blogs.nature.com\/methagora\/2009\/08\/top_downloads_for_july_09_1.html","title":{"rendered":"Top downloads for July ’09"},"content":{"rendered":"

Two Correspondences made the list of top downloads for July coming in at #3 and #4, demonstrating that while this format may not report new methods it does have information of high interest to readers. The two top downloads seem to highlight a high level of interest in assaying single cells and using FRET<\/span> to examine protein dynamics.<\/p>\n

Top 8 research papers published in the July issue<\/u><\/strong><\/p>\n

1. Quantitative analysis of gene expression in a single cell by qPCR<\/a><\/p>\n

2. Mapping the structure and conformational movements of proteins with transition metal ion FRET<\/span> <\/a><\/p>\n

3. Limitations and possibilities of small RNA<\/span> digital gene expression profiling<\/a><\/p>\n

4. Enabling IMAC<\/span> purification of low abundance recombinant proteins from E. coli lysates<\/a><\/p>\n

5. In vivo fluorescence imaging with high-resolution microlenses<\/a><\/p>\n

6. Protein interaction platforms: visualization of interacting proteins in yeast<\/a><\/p>\n

7. Agouti C57BL\/6N embryonic stem cells for mouse genetic resources<\/a><\/p>\n

8. Reaching the protein folding speed limit with large, sub-microsecond pressure jumps<\/a><\/p>\n

There has been very little movement in the ten most popular papers published prior to our July issue and downloaded during July. The exception is the appearance of the HUPO<\/span> test sample paper from June appearing at #7. The papers below this have changed but mostly because a large number of papers have very similar download numbers and they shuffle around from month to month.<\/p>\n

Top 10 research papers published prior to the July issue<\/u><\/strong><\/p>\n

1. Mapping and quantifying mammalian transcriptomes by RNA<\/span>-Seq<\/a><\/p>\n

2. mRNA-Seq whole-transcriptome analysis of a single cell<\/a><\/p>\n

3. Universal sample preparation method for proteome analysis<\/a><\/p>\n

4. Stem cell transcriptome profiling via massive-scale mRNA sequencing<\/a><\/p>\n

5. Isolation of human iPS cells using EOS<\/span> lentiviral vectors to select for pluripotency<\/a><\/p>\n

6. Genome-wide profiles of STAT1 DNA<\/span> association using chromatin immunoprecipitation and massively parallel sequencing<\/a><\/p>\n

7. A HUPO<\/span> test sample study reveals common problems in mass spectrometry\u2013based proteomics<\/a><\/p>\n

8. Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon<\/a><\/p>\n

9. Amplification-free Illumina sequencing-library preparation facilitates improved mapping and assembly of (G+C)-biased genomes<\/a><\/p>\n

10. A versatile tool for conditional gene expression and knockdown<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Two Correspondences made the list of top downloads for July coming in at #3 and #4, demonstrating that while this format may not report new methods it does have information of high interest to readers. The two top downloads seem to highlight a high level of interest in assaying single cells and using FRET to examine protein dynamics.  Read more<\/a> Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":98,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-65","post","type-post","status-publish","format-standard","hentry","category-nature-methods-papers"],"_links":{"self":[{"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/posts\/65","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/users\/98"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/comments?post=65"}],"version-history":[{"count":0,"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/posts\/65\/revisions"}],"wp:attachment":[{"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/media?parent=65"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/categories?post=65"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.nature.com\/methagora\/wp-json\/wp\/v2\/tags?post=65"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}