Nature Protocols | Stepwise

Old and new protocols on stable isotope labelling of proteins for mass spec

On Thursday last week, we published a protocol for spike-in SILAC, a method to analyse the proteome of tissues and organisms developed by Yasushi Ishihama & Matthias Mann and co-workers. This is one of many protocols that we have published that uses stable isotope labelling of proteins or peptides prior to separation and analysis by mass spectrometry. A list of these can be found here. 

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