{"id":222,"date":"2012-03-21T16:36:05","date_gmt":"2012-03-21T16:36:05","guid":{"rendered":"https:\/\/blogs.nature.com\/stepwise\/?page_id=222"},"modified":"2012-03-21T16:36:05","modified_gmt":"2012-03-21T16:36:05","slug":"single-cell-analysis-techniques","status":"publish","type":"page","link":"https:\/\/blogs.nature.com\/stepwise\/single-cell-analysis-techniques","title":{"rendered":"Single-cell analysis techniques"},"content":{"rendered":"

Here we present a collection of just\u00a0a few\u00a0of our\u00a0single-cell analysis protocols for your interest, in advance of\u00a0our attendance at the upcoming Select Biosciences Single Cell Analysis Europe<\/span><\/a> conference in Edinburgh from the 28th-29th March.\u00a0<\/p>\n

As you will see,\u00a0the methods range from gene expression and copy number profiling to isolation and imaging of single cells, as examples of the content we have published on this topic over the years.\u00a0<\/p>\n

These\u00a0protocols will be free to access until the end of March\u00a0and we hope you will\u00a0find them useful!<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u00a0\"\"<\/td>\nMicroarray analysis of copy number variation in single cells<\/a><\/strong>
\nKonings et al.<\/em>
\nThis protocol enables reliable detection of\u00a0DNA copy number aberrations in a single cell,\u00a0by hybridizing\u00a0multiple displacement-amplified DNA to BAC- and SNP-arrays.\u00a0\u00a0 \u00a0<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nComprehensive qPCR profiling of gene expression in single neuronal cells<\/a><\/strong>
\nCitri et al.<\/em>
\nThis protocol\u00a0describes a single-cell assay to characterize lineage-specific reprogrammed human neuronal cells, using qPCR gene expression profiling.\u00a0\u00a0<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nMeasuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy<\/a><\/strong>
\nYoung et al.<\/em>
\nQuantitative single-cell time-lapse microscopy forms the basis of this protocol, enabling\u00a0gene expresion and regulation to be quantified in individual E. coli <\/em>cells.\u00a0\u00a0<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nRapid isolation of single malaria parasite\u2013infected red blood cells by cell sorting<\/a><\/strong>
\nMiao & Cui
\nIn this protocol, a method for efficient cloning of the malaria parasite is presented that\u00a0uses a cell sorter to rapidly isolate single P. falciparum<\/em>\u2013infected red blood cells.\u00a0\u00a0<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\n\n

Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy<\/a><\/strong>
\nFriedrichs, Helenius & Muller
\nHere, a protocol to study integrin-mediated adhesion of HeLa cells to collagen type I using atomic force microscopy-based single-cell force spectroscopy is presented.\u00a0<\/p>\n<\/td>\n<\/tr>\n

\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nRNA-Seq analysis to capture the transcriptome landscape of a single cell<\/a><\/strong>
\nTang et al.<\/em>
\nThis digital transcriptome analysis protocol\u00a0uses a deep-sequencing approach to analyze the functional readout of the genome from\u00a0a single mouse oocyte.<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nTargeted single-cell electroporation of mammalian neurons in vivo<\/em><\/a><\/strong>
\nHausser et al.<\/em>
\nThis protocol for single-cell electroporation\u00a0provides a method for delivering plasmid DNA into single identified mammalian neurons in vivo<\/em>.<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nSingle-cell isolation from cell suspensions and whole genome amplification from single cells to provide templates for CGH analysis<\/a><\/strong>
\nGiegl & Speicher
\nHere, the authors describe various options for\u00a0single-cell isolation and whole genome amplification, allowing a comprehensive genomic analysis of single cells.<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nGlobal single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis<\/a><\/strong>
\nKurimoto et al.<\/em>
\nThe representative amplification of global mRNAs from typical single mammalian cells is achieved using this protocol,\u00a0providing a\u00a0template for microarray analysis.<\/td>\n<\/tr>\n
\u00a0<\/td>\n\u00a0<\/td>\n<\/tr>\n
\"\"<\/td>\nAdult mouse hematopoietic stem cells: purification and single-cell assays<\/a><\/strong>
\nEma et al.<\/em>
\nIn this protocol, the authors present their\u00a0single-cell colony assay and single-cell transplantation procedures to characterize purified haematopoietic stem cells.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Here we present a collection of just\u00a0a few\u00a0of our\u00a0single-cell analysis protocols for your interest, in advance of\u00a0our attendance at the upcoming Select Biosciences Single Cell Analysis Europe conference in Edinburgh from the 28th-29th March.\u00a0 As you will see,\u00a0the methods range … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":111,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-222","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/pages\/222","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/users\/111"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/comments?post=222"}],"version-history":[{"count":0,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/pages\/222\/revisions"}],"wp:attachment":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/media?parent=222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}