{"id":447,"date":"2012-07-13T11:53:03","date_gmt":"2012-07-13T11:53:03","guid":{"rendered":"https:\/\/blogs.nature.com\/stepwise\/?p=447"},"modified":"2012-07-13T12:58:39","modified_gmt":"2012-07-13T12:58:39","slug":"top-exchange-protocols-by-number-of-views","status":"publish","type":"post","link":"https:\/\/blogs.nature.com\/stepwise\/2012\/07\/13\/top-exchange-protocols-by-number-of-views","title":{"rendered":"Top Exchange Protocols – By number of views"},"content":{"rendered":"

The three most viewed Exchange Protocols appear on the Protocol Exchange homepage<\/a>; they don’t seem to change much, mostly because they are always highlighted!<\/p>\n

This blogpost will list the top 13 Exchange Protocols (it is after all the 13th of July!) to give a better picture of which protocols people find most interesting, and to show our appreciation for some the researchers who upload excellent protocols to our site.<\/p>\n

Of course, that they are the most downloaded does not necessarily mean that they are the ones that will be most useful to you. To find protocols relevant to your research, try using the browse function<\/a>\u00a0on our site.<\/p>\n

The list below was obtained using Webtrends<\/a>, and reflects the order of protocols in terms of the number of views over the last 91 days.<\/p>\n

 <\/p>\n

1. \u00a0A protocol for in vivo detection of reactive oxygen species<\/a><\/p>\n

2.\u00a0Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation<\/a><\/p>\n

3. \u00a0Production of neuron-preferential lentiviral vectors<\/a><\/p>\n

 <\/p>\n

4. \u00a0Western blot analysis of sub-cellular fractionated samples using the Odyssey Infrared Imaging System<\/a><\/p>\n

Yukiko Misawa, Ying Li, David Rekosh & Marie-Louise Hammarskjold<\/p>\n

5. \u00a0Fluorescence in situ hybridization (FISH) for DNA replication origins<\/a><\/p>\n

Patrizia Gasparini & Omar Malazzi<\/p>\n

6. \u00a0COBRA Toolbox 2.0<\/a><\/p>\n

Daniel Hyduke et al.<\/em> from Bernhard Palsson’s lab<\/p>\n

7. \u00a0Trans-well migration assay<\/a><\/p>\n

Ofer Mandelboim<\/p>\n

8.\u00a0Measuring fatty acid oxidation in tissue homogenates<\/a><\/p>\n

Matthew D Hirschey & Eric Verdin<\/p>\n

9. \u00a0Chromatin immunoprecipitation (ChIP) assay<\/a><\/p>\n

Zhongfu Ni, Danny W.-K. Ng, Jianxin Liu & Z.Jeffrey Chen<\/p>\n

10. \u00a0Combination of chemical cross-linking and pull-down assay to study transient protein-protein interactions<\/a><\/p>\n

Feng Gong, Deirdre Fahy & Michael J. Smerdon<\/p>\n

11. \u00a0Immunohistochemistry and in situ hybridization protocols<\/a><\/p>\n

Joshua Hunsberger & Samuel Newton<\/p>\n

12. \u00a0Simultaneous detection of murine antigen-specific intracellular cytokines and CD107a\/CD107b by flow cytometry<\/a><\/p>\n

Joyce J. Ruitenberg, Smita A. Ghanekar, Dirk G. Brockstedt \u00a0& Holden T. Maecker<\/p>\n

13.\u00a0A simple and rapid method for RNA isolation from plant tissues with high phenolic compounds and polysaccharides<\/a><\/p>\n

Kam-Lock Chan, Chai-Ling Ho, Parameswari Namasivayam & Suhaimi Napis<\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

The three most viewed Exchange Protocols appear on the Protocol Exchange homepage; they don\u2019t seem to change much, mostly because they are always highlighted!  Read more<\/a> Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":216,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[41,1],"tags":[],"class_list":["post-447","post","type-post","status-publish","format-standard","hentry","category-spotlights","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/posts\/447","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/users\/216"}],"replies":[{"embeddable":true,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/comments?post=447"}],"version-history":[{"count":0,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/posts\/447\/revisions"}],"wp:attachment":[{"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/media?parent=447"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/categories?post=447"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blogs.nature.com\/stepwise\/wp-json\/wp\/v2\/tags?post=447"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}