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Membrane protein protocols

Membrane protein protocols

While browsing recently published papers in other journals, I came across Supramolecular fishing for plasma membrane proteins using an ultrastable synthetic host-guest binding pair (published in Nature Chemistry). The idea here is that proteins on the surface of the cell are chemically modified by reaction with 1-trimethylammoniomethylferrocene (AFc). After cell lysis, the modified proteins bind to beads coated with cucurbit-7-uril. Cucurbit-7-uril and AFc interact to form an ultrastable host-guest binding pair. The captured proteins are then recovered either by treatment with a strong competitor or by heating to 95 C in buffer conditions.  Read more

Old and new protocols on stable isotope labelling of proteins for mass spec

Old and new protocols on stable isotope labelling of proteins for mass spec

On Thursday last week, we published a protocol for spike-in SILAC, a method to analyse the proteome of tissues and organisms developed by Yasushi Ishihama & Matthias Mann and co-workers. This is one of many protocols that we have published that uses stable isotope labelling of proteins or peptides prior to separation and analysis by mass spectrometry. A list of these can be found here.  Read more