PRIDE Converter – A new tool for proteomics

The administrators of the EBI proteomics repository PRIDE have just announced, in the July issue of Nature Biotechnology, the availability of a software tool to facilitate data deposition.

According to Lennart Martens and co-authors, the new tool, PRIDE Converter, “makes it straightforward to submit proteomics data to PRIDE from most common data formats.” This comes handy.

Depositing proteomics data into a structured public repository is a very worthwhile effort—one that Nature Biotechnology, Nature Methods, and other Nature journals strongly encourage. To date, however, the problem has been that in some cases, depositing data remained an effort.

Things could get particularly challenging if you happened to have large datasets in instrument-specific formats. Converting these into the XML-based format needed for data submission required time and informatics skills. Apparently, now, PRIDE Converter does it for you.

Proteomics researchers need effective ways of sharing data. Submission of data to public repositories upon publication should become as automatic in proteomics as it is in some other fields. But realistically, this can only happen if a good infrastructure of databases is in place—it is—and if the submission process is not an undue burden on the researchers.

Kudos to PRIDE, thus, for taking this step in the right direction and for demonstrating a willingness to work with researchers to facilitate submission. It is up to proteomics researchers now, to make use of the tool, and work with its authors to continuously improve it as the field, and their needs, evolve.

Data overload

How do you handle terabytes of data? That is a question that more and more investigators must face, on a weekly basis.

Are you one of them? Light-sheet fluorescence imaging, for example, generates so much data in each experimental run that handling and storing the raw data is a challenge. Next-generation sequencing is another, much more ubiquitous, case.

Read the July issue editorial “Byte-ing off more than you can chew” and let us know about your own experience, problems and practical (or impractical) solutions.

Focus on single-molecule analysis

In June, Nature Methods focuses on the latest trends in single-molecule analysis.

Leaders in the field review the methodologies allowing to examine the mechanism of action of fundamental biological processes molecule by molecule. The Review and Perspective articles also provide practical guidance for some of the techniques that are most integral to in vitro single-molecule experiments.

They cover single-molecule fluorescence methods, microfluidic flow cells to manipulate experimental conditions during experiments, and force spectroscopy techniques such as atomic force and optical traps to manipulate individual molecules.

In addition, two timely original research articles in the same issue describe complementary advances in nanoscale visualization.

All these articles are accessible free of charge for the entire month of June. Check them out!

Compare and conquer

Experimental comparisons of methods, technology platforms or reagents are time-consuming and expensive, but hugely beneficial. An Editorial in the April issue of Nature Methods illustrates how such comparisons have been very useful for scientists in various research disciplines. Nature Methods has now adopted an article format called ‘Analysis’ to accommodate publication of such comparative analyses. Broad guidelines of what the editors will be looking for are provided in the April Editorial.

Method of the Year

Nature Methods has named next-generation sequencing its inaugural Method of the Year.

Check out the special feature (freely accessible online) with Commentaries by Stephan Schuster and by Barbara Wold and Rick Myers. In addition to celebrating the Method of the Year 2007 the special feature contains a shortlist of Methods to Watch in the years to come.

Here, we welcome your comments on our choices as well as your suggestions of other methods to keep an eye on. We firmly intend this event to become an end-of-the-year tradition, and we hope for your participation in next year’s nominations!

Research in situ, where it is most needed.

The Global Theme Issue on Poverty and Human Development went live today: 235 scientific journals coordinated the publication of articles specifically addressing issues related to human condition in poverty-stricken areas. This initiative was spearheaded by the Council of Science Editors and the full list of articles can be found on their web site. A special event was hosted at the US NIH on the occasion of the coordinated publication.

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No faulty-gene carrier need apply

A bill designed to prohibit discrimination based on genetic information in terms of health insurance and employment is awaiting a vote in the US Senate. There have been several similar but ultimately unsuccessful legislative attempts over the past 12 years. It is crucial that this bill be enacted into law to address a public fear likely to limit patient access to predictive genetic testing and to discourage participation in genetic research.

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Staff picks

In an editorial in the July issue, we suggest some recent popular science books that would make a nice summer read and hope that summer will not be the end of it. To keep you going, here are an extended list of older books, including some of our favorites and some of the genre classics. The list was assembled with the kind advice of editors, scientists and science writers.

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