Bryan Dickinson is in the Department of Chemistry at The University of Chicago, and works on developing both small molecule and synthetic biology technologies to measure and control chemistry in living cells. Bryan recently published a paper entitled “A fluorescent probe for cysteine depalmitoylation reveals dynamic APT signaling” in Nature Chemical Biology.

1. What made you want to be a chemist?

As I explored different types of science through courses and research experiences in college, I found chemistry approaches science with what I believe is a perfect balance of molecular understanding and real-world applicability. Our ability as chemists and bioengineers to build molecules that actually do something continues to fascinate me, and is the ultimate motivation behind every project I pursue.

2. If you weren’t a chemist and could do any other job, what would it be — and why?

One of my current hobbies is woodworking, both art pieces and furniture. If I could build all day I think I would be pretty happy. I like crafting something with my hands that never existed before and would not have except for my hard work. In that regard, research in my lab is quite similar. Whether your building a table, synthesizing a small molecule, or engineering a protein, success comes from focus, persistence, and creativity. The latter is especially important to me, because in design and art, as well as technology, I find beauty in creative approaches that impart functionality.

3. What are you working on now, and where do you hope it will lead?

Two project areas in the group just had some nice breakthroughs. In one area, we came up with a very general small molecule strategy to measure cysteine PTM “eraser” enzymes in live cells and used it to discover that cysteine lipidation status is dynamically regulated. We hope these tools will lead to a better understanding of how cysteine PTMs are regulated, and how alterations in that regulation effect cell physiology. In a second project area, we just developed a split RNAP-based biosensor engineering platform. This approach is opening up a variety of new directions in my group, including new evolution systems, mammalian synthetic biology tools, and new ways to measure endogenous biochemistry.

4. Which historical figure would you most like to have dinner with — and why?

I would love to have dinner with Rosalind Franklin, who was actually my current age (32) when she gathered the first X-ray diffraction image of DNA. That single image lead to the elucidation of the double helix structure of DNA and the discovery of the biophysical basis for the central dogma. She of course tragically passed away just five years after gathering that data. I imagine a dinner with Rosalind would be immensely interesting, during which she could explain what it was like to be a scientist during the beginnings of modern molecular biology, and maybe reveal how she was able to be so successful at such a young age while facing so many challenges. Moreover, I would really enjoy explaining to her what we can do with DNA today in terms of synthetic biology, which I imagine would sound like science fiction.

5. When was the last time you did an experiment in the lab — and what was it?

I actually really enjoy being in lab and try to help with experiments as much as possible. Most of the time I do the stuff in lab that no one else wants to do, like making cell media and molecular biology reagents. Last week I assisted with a confocal imaging experiment, which was lots of fun. When I have a few extra minutes, I enjoy designing primers for new cloning projects, which I am still quite good at. I refer to members of my team that surpass me in their cloning abilities as “cloning ninjas”, of which I have a few now, but I am still the “cloning sensei” and work hard to keep that rank.

6. If exiled on a desert island, what one book and one music album would you take with you?

The Great Gatsby is the one book I can read over and over again and still enjoy. Music is a tough one to choose. I think if I were exiled to an island by society for some reason that I’d be feeling pretty angsty, and Green Day’s Dookie has been one of my go-to soundtracks for that mood since I was in third grade.

7. Which chemist would you like to see interviewed on Reactions — and why?

I would like to see Carolyn Bertozzi interviewed. She has a great perspective on the field of chemistry and is always full of excellent advice, especially for young scientists.

{credit}Bryce Vickmark, MIT{/credit}

Ed Boyden is in the MIT Media Lab and McGovern Institute, and a professor in the Departments of Biological Engineering and Brain and Cognitive Sciences, where he directs a group that develops and applies new molecular and optical tools to neural circuit analysis. Ed recently published a paper entitled “Engineering genetic circuit interactions within and between synthetic minimal cells” in Nature Chemistry.

1. What made you want to be a chemist?

Even as a kid, I was very philosophical — wondering what was the meaning of life, and what it meant to be human, and what was the nature of consciousness. At age 14, I entered a program at the University of North Texas that let students directly begin college instead of finishing high school. I joined Prof. Paul Braterman’s chemistry lab as a research assistant, working on a project to create the building blocks of life from scratch. I worked on a project where we tried to insert ferrocyanide into layered double hydroxides, and then to drive reactions that would convert ferrocyanide into nucleic acids like adenine. Immediately this struck me as amazing — a philosophically interesting experiment, which was at its heart a chemical project, and chemistry would give you the tools to confront it.

2. If you weren’t a chemist and could do any other job, what would it be — and why?

After two years at the University of North Texas, I transferred to MIT, where I completed degrees in electrical engineering and computer science, and physics. At that point I felt like I completed my “skill” training, and then decided it was time to pick some hard problems work on, and that was the point where I switched into neuroscience, entering the Stanford neuroscience PhD program where I worked in the labs of Richard Tsien and Jennifer Raymond on learning and memory (and, in parallel, co-launched optogenetics with Karl Deisseroth, as a side project done in parallel to my PhD). So I would have to say that I’m not only a chemist, although the focus of my group is largely on molecular strategies for understanding biological complexity. I would have to say that I think backwards from problems, try to survey every possible angle of attack on them (whether chemical, physical, electrical, or computational), and then take the best approach. Much of the time, that does mean a molecular tool, but we also do work in robotics, optics, and computational modeling. So I would have to say I’d want to continue to be a problem solver, tackling problems with whatever discipline is the best one for the job. If I had to leave science entirely, though, I’d probably want to write novels or movie screenplays — I like to write.

3. What are you working on now, and where do you hope it will lead?

Right now, we are working on a way to image biological specimens, like cells and tissues, in 3D with nanoscale resolution. We achieve that by synthesizing a polyelectrolyte gel evenly throughout a preserved specimen, anchoring key biomolecules or labels to the specimen, and then adding water so the polyelectrolyte gel swells and takes the biomolecules along for the ride. The net effect is like drawing a picture on a balloon and then blowing up the balloon — except in 3-D. We call the technology expansion microscopy. The idea of embedding a preserved tissue in a hydrogel is an old one, dating perhaps back to 1995¹. In 2015 we showed the basic concept of expansion microscopy². Then in 2016 we revealed powerful and easy protocols for analyzing proteins³  and nucleic acids⁴. The net impact is that people can map the identity and locations of biomolecules through complex systems like brain circuits, cancer biopsies, and so forth — helping reveal how molecules are configured to implement biological processes, and how they go wrong in disease states. I hope that a wealth of biological insight, as well as better disease targets, will emerge.

4. Which historical figure would you most like to have dinner with — and why?

Hmm… probably Archimedes. First, we just don’t know much about him, at least not compared to modern scientists — much about him remains cloaked in myth or legend. Second, he anticipated quite a bit of mathematics and physics that followed him, and was in some ways far ahead of his time. And developed many practical inventions. It would be great to find out what his influences were, and how he thought about his culture and time.

5. When was the last time you did an experiment in the lab — and what was it?

For the first few years I was a professor at MIT, I often would teach students how to do experiments — preparing cells with optogenetic tools expressed, performing patch clamp electrophysiology, aligning optics — and then do experiments side-by-side with them. The last set of experiments I did from start to finish, that resulted in a paper, were the optogenetics experiments for the 2005 paper that kicked off the modern excitement about controlling neurons with light⁵.

6. If exiled on a desert island, what one book and one music album would you take with you?

I suppose How to Survive on a Deserted Island wouldn’t really count? (Apparently it is a real book, and has at least some good reviews.) So I suppose it should be a really long book, and a really long album. (As opposed to a life-changing book or album that might have such impact, that reading it or hearing it too often becomes unnecessary or even tiresome.) So maybe for the book, I’d pick the Encyclopedia Brittanica (if you can still buy that). For an album — I never seem to tire of Chopin’s piano works, so maybe a collection of those.

7. Which chemist would you like to see interviewed on Reactions – and why?

A living chemist, or any chemist ever? If the latter, it could be interesting to interview Lavoisier as it seems like he had an interesting life. (Lots of chemists did, it seems.) If living, there are so many great folks who could be fun to interview, but if I just had to pick one, how about Ferran Adrià, a chef who is one of the pioneers of bringing chemistry-driven techniques into modern cooking?

[1] Microscopy Research and Technique 30, 513–520 (1995)

[2] Science 347, 543–548 (2015).

[3] Nature Biotechnology 34, 987–992 (2016)

[4] Nature Methods 13,679–684 (2016)

[5] Nature Neuroscience 8, 1263–1268 (2005)

Katherine Mirica is an Assistant Professor in the Department of Chemistry at Dartmouth College. The Mirica group works on the design and synthesis of stimuli-responsive materials for portable chemical sensing and microelectronics.

1. What made you want to be a chemist?

While I grew up among chemists (my mother is a chemist) in a small industrial town of Ukraine, and enjoyed chemistry in high school, it was not until my involvement in research during my sophomore year at Boston College that I had solidified my decision to study chemistry. I became fascinated with organic and materials chemistry and the potential of these fields in solving global challenges in healthcare.

2. If you weren’t a chemist and could do any other job, what would it be — and why?

An architect — I’ve always loved designing and making things.

3. What are you working on now, and where do you hope it will lead?

My group focuses on the design of conductive multifunctional nanomaterials for gas-phase sensing to enable portable and personalized diagnosis and monitoring of diseases. We are particularly interested in designing sensors for a class of molecules known as gasotransmitters (e.g., nitric oxide, carbon monoxide, and hydrogen sulfide), to attain fundamental understanding of the role of these physiological modulators in human disease. Materials and methods developed in the group may also be applicable to information storage, and energy storage, conversion, and catalysis.

4. Which historical figure would you most like to have dinner with — and why?

Richard Feynman. His vision for nanotechnology at the time was extraordinary, and his ability to communicate complex concepts with such clarity was truly remarkable. I regularly watch videos of him on YouTube, and greatly admire both his work and ability to question and explain things.

5. When was the last time you did an experiment in the lab — and what was it?

I did a sensing experiment for my first paper as an independent investigator, which was recently published in Chemistry of Materials.

6. If exiled on a desert island, what one book and one music album would you take with you?

The book would have to be War and Peace by Leo Tolstoy (in original Russian), and the music would be Four Seasons by Antonio Vivaldi. One is bound to discover new meaning in these classics every time they are revisited.

7. Which chemist would you like to see interviewed on Reactions — and why?

My PhD advisor, George Whitesides. I bet he would have very interesting answers.